Fig 5. Upregulation of autophagy by Atg3 overexpression increases the sensitivity of SKM-1 cells to bortezomib treatment.

(A) Annexin V-APC/ PI double staining flow cytometry of each group treated with increasing concentrations of bortezomib (1 nM, 10 nM, 50 nM, and 100 nM). (B) The PI positive rate of each group with the indicated concentration of bortezomib. (C) Cellular nuclei were stained by DAPI and visualized using fluorescence microscopy at 24 h after 10 nM bortezomib treatment. (D) Cell viability of each group was analyzed by CCK-8 assay in the absence or presence of 10 nM bortezomib for 24 h. All data are representative of three independent experiments and are shown as the mean±SD. *p<0.05, **p<0.01, ***p<0.001.