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. 2016 Jul 8;11(7):e0158980. doi: 10.1371/journal.pone.0158980

Fig 3. General walking pattern: 2D representative schemes.

Fig 3

Gait patterns registered 3 h after the mice had received i.m. injection of saline or B. jararacussu snake venom into the right gastrocnemius. (A) Mice exhibited a normal gait pattern, with paw imprints of the saline injected right hindlimb (RH, red arrows) perfectly delineated on the captured image; (B) Respective 2D footprint graphic representing maximum intensity of the right hindlimb (RH) footprint register (black arrow), as well as were also normally imprinted the right frontlimb (RF), left hindlimb (LH) and right frontlimb (LF), thus indicating that at 3 hours after injection/manipulation an even RH gait was preserved at every run. (C) RH footprint was absent in the lane what was confirmed by the 2D software footprint register (D), thus indicating that mice show zero intensity of RH footstep 3 hours after Bjssu injection into the right gastrocnemius (blue arrow). Notice that the intensity of the footstep of LH, LF and RF is higher, since the area of the pad and fingers appears strongly delineated relative to what is seen in the images of animals injected with saline.