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. 2016 Jul 8;11(7):e0158777. doi: 10.1371/journal.pone.0158777

Fig 2. Expression of MMP regulation pathway-related genes in aged gingival tissues.

Fig 2

(A) RNA sequencing of MMP regulation-related genes in young and old gingival tissues (n = 3 per group). Red and green stars indicate mRNA that were up- and downregulated, respectively, in old gingival tissues. Red color indicates high-level gene expression in young or old gingival tissues. (B) Confirmation of RNA sequencing results using real-time PCR (n = 5 per group; *p < 0.05). (C) MMP activity was assessed by zymography. (D and G) Total MMP activity (D) and specific MMP activities (G) were calculated according to the density of each protein band. Y, young gingival tissues; and O, old gingival tissues.