Table 1. Ki and αKi values and inhibition patterns of PEPCK-C isoenzymes in presence of 3-MP.
| PEP | GDP | OAA | GTP | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ki (μM) | αKi (μM) | Pattern of inhibition | Ki (μM) | Pattern of inhibition | Ki (μM) | αKi (μM) | Pattern of inhibition | Ki (μM) | Pattern of inhibition | |
| 139Met | 16 ± 2 | 20 ± 4 | Mixed NC | 45 ± 3 | Uncompetitive | 18 ± 4 | 49 ± 18 | Mixed NC | 273 ± 68 | Uncompetitive |
| 139Leu | 19 ± 4 ns | 31 ± 5 ns | Mixed NC | 45 ± 0.8 ns | Uncompetitive | 29 ± 1 * | 21 ± 13 ns | Mixed NC | 873 ± 144 ** | Uncompetitive |
Values given are the mean ± SD. Differences were calculated using two-sample t-test; ns: no significant,
* p< 0.05,
** p< 0.01.
NC: Non-Competitive