Figure 1.

H1-3XFLAG is functional and binds to chromatin. (A) A cartoon illustrating the strategy for introducing a 3x-flag sequence into the 3′ end of the native hH1 locus by homologous recombination is shown. (B) The linear growth rate of the indicated strains was measured using ‘race tubes’ for three replicates of each strain. The direction of growth is left to right. (C) Quantification of the linear growth rate data from panel B as distance (y-axis; cm) vs. time (x-axis; hr). The three lines shown for each genotype represent three independent race tubes. (D) The soluble (Sol) and the chromatin fractions (Chr) were isolated from wild-type and the hH1-3xflag-Hph+ strain, and both fractions were analyzed by western blotting using anti-FLAG and anti-H3 antibodies, as indicated. MW, positions and sizes in kDa of a prestained protein ladder; WT, wild-type.