Table 3. Repression of gonadal dysgenesis in the daughters of TP6 y+ w/y w; hobo/+ females from reciprocal crosses between TP6 y+ w and y w; hobo strains.
Neithera | Transgene Onlya | TP6 Onlya | Botha | Transgene Absentb | Transgene Presentb | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transgenic Stock | Cross | No. of Vials | No. of Flies | %GD ± SEc | No. of Flies | %GD ± SEc | No. of Flies | %GD ± SEc | No. of Flies | %GD ± SEc | No. of Flies | %GD ± SEc | No. of Flies | %GD ± SEc | Comparison Scored |
None | A | 25 | 260 | 97.8 ± 1.3 | 246 | 98.0 ± 0.9 | 506 | 97.7 ± 1.1 | |||||||
H(hsp/KP)7 | A | 15 | 67 | 73.8 ± 6.7 | 66 | 25.7 ± 9.3 | 60 | 75.8 ± 7.6 | 49 | 11.4 ± 5.6 | 127 | 74.9 ± 5.1 | 115 | 18.5 ± 6.0 | 15* |
H(w+, KP1)C | A | 20 | 134 | 95.6 ± 2.3 | 116 | 60.4 ± 4.0 | 131 | 95.7 ± 2.0 | 92 | 60.8 ± 6.7 | 265 | 96.0 ± 1.5 | 208 | 58.9 ± 3.5 | 20* |
H(w+, KP1)J | A | 27 | 86 | 99.4 ± 0.6 | 210 | 75.4 ± 3.9 | 68 | 97.2 ± 1.9 | 193 | 72.4 ± 4.9 | 154 | 98.5 ± 0.8 | 403 | 74.3 ± 3.5 | 24* |
None | B | 19 | 173 | 99.1 ± 0.9 | 170 | 98.2 ± 1.2 | 343 | 98.7 ± 1.0 | |||||||
H(hsp/KP)7 | B | 13 | 58 | 100 ± 0 | 53 | 36.8 ± 9.1 | 49 | 96.2 ± 2.8 | 68 | 30.3 ± 7.0 | 107 | 98.3 ± 1.1 | 116 | 35.7 ± 5.6 | 13* |
H(w+, KP1)C | B | 26 | 185 | 98.4 ± 1.6 | 137 | 91.9 ± 3.2 | 148 | 100 ± 0 | 128 | 92.1 ± 2.3 | 333 | 99.0 ± 1.0 | 265 | 91.6 ± 2.1 | 12 |
H(w+, KP1)J | B | 13 | 24 | 100 ± 0 | 82 | 86.3 ± 3.9 | 25 | 100 ± 0 | 68 | 93.5 ± 2.9 | 49 | 100 ± 0 | 150 | 88.7 ± 2.9 | 10 |
Indicates numbers that are statistically significant by the sign test. GD, gonadal dysgenesis; A, crosses using TP females; B, crosses using transgenic females.
Two factors—the telomeric element (TP6) and the hobo transgene—segregated in the test crosses, giving rise to four genotypic classes in the F2. The headings indicate which of these two factors were present in the females that were scored for GD.
The four categories of data have been pooled into two categories to assess the effect of the transgene in the F2 females that were scored for GD.
Unweighted average percentage GD ± SE.
Number of vials in which the F2 females carrying the transgene had a lower GD frequency than the females not carrying it.