Figure 1.

Summary of bead method. (A) Diagram of C. elegans life history choice. Continuous development (left) occurs in favorable environmental conditions, whereas dauer development (right) occurs in adverse environmental conditions. The boxed region is shown in more detail on the far right. Horizontal lines represent molts. (B) Summary of the method used in this paper. Lower magnifications are possible for older larvae or adults. (C) Representative images of L4 (intermolt) and L4 molt staged hermaphrodites on bead-containing plates. This image was taken on a Leica MZ16F dissecting microscope with a DS-Red filter using a 1 × objective and 10 × zoom to produce 100 × magnification. Scale bar = 0.1 mm. L, larval stage; OP50, OP50 strain of E. coli; PD, postdauer.