Figure 2.

Visualizing SNPs present in five or fewer TM3 chromosomes reveals numerous single crossover events on 3L and several DCO events on 3R (see Materials and Methods). Blue lines indicate the positions of inversion breakpoints whose precise location is known, orange dashed lines show the approximate positions of the unidentified In(3R)C (92D1-E1;100F2-3) inversion breakpoints. Gray shaded regions are centromere-proximal heterochromatin with low-quality read alignment. (A) Single crossovers are common in the region distal to the 65D inversion breakpoint at position 3L:6,925,034, and occur no closer than 2 Mb from the breakpoint. (B) Several DCOs are apparent on 3R. Stocks TM3-560 and TM3-1614 may be versions of TM3 before Ser¹ was added to a TM3, Sb⁺ Ser⁺ chromosome (TM3-560), and before Sb¹ was added to a TM3, Sb⁺ Ser¹ chromosome (TM3-1614).