Figure 1.

Mutation accumulation (MA) experiments in pico-algae. Flow cytometer measurements were performed every 14 d to make one cell bottlenecks for each line. The ancestral culture of each species came from one single cell, inoculated in a well to grow enough cells to start the experiment. The ancestral culture was maintained with higher effective population size in the control lines (inoculation of 100 cells) and MA lines by reinoculating one single cell, in six replicates per line, in 24-well microtiter plates.