Table 3. Genes selected for real-time PCR validation and the fold change in the postlarval stage (Y5) compared with the four-spiral whorls stage (competent larvae J4) for both RNA-seq and RT-PCR analysis.

			log2.fold_change (Y5 vs. J4)
Gene ID	Gene Name	Exp	RNA-Seq	RT-PCR	Same Trend
c95470_g1	vdg3	↓	−9.55**	−6.00**	Y
c131243_g1	CTSV	↓	−8.40**	−8.90*	Y
c139260_g1	CAV1	↓	−7.39**	−1.84**	Y
c129336_g1	Fcer2	↓	−6.30**	−7.46**	Y
c125484_g1	SARP-19	↓	−5.75**	−2.01**	Y
c128565_g1	CA2	↓	−5.63**	−4.23*	Y
c145993_g2	ACT1	↓	−5.02**	−1.91*	Y
c127784_g1	Lrp1	↓	−4.71**	−4.06**	Y
c145412_g1	nas-14	↓	−4.59**	−4.35*	Y
c119967_g1	CRISPLD2	↓	−4.29**	−2.67*	Y
c150152_g1	ENTPD3	↑	4.56**	8.20*	Y
c113545_g2	WBSCR27	↑	4.50**	5.73**	Y
c94349_g1	pxdn	↑	4.49**	5.50*	Y
c156029_g2	xynA	↑	4.43**	7.30**	Y
c117375_g2	TNF	↑	4.38**	7.79**	Y
c147483_g1	Lrp4	↑	4.34**	6.44*	Y
c149073_g1	Arsj	↑	4.31**	8.56**	Y
c143535_g1	LCP1	↑	3.89**	4.33**	Y
c135964_g1	IRX12	↑	3.85**	6.37**	Y
c118662_g1	Lrp2	↑	3.81**	4.34**	Y

Genes with the same trend (up or downregulation) as shown by both RNA-seq and RT-PCR analysis are indicated by “Y” (yes). * P < 0.05, ** P < 0.01 (significant difference between J4 and Y5 stages). Exp, expression trend; “↑”upregulated expression; “↓” downregulated expression.