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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Structure. 2016 Jun 2;24(7):1068–1080. doi: 10.1016/j.str.2016.04.015

Figure 7. Positive difference density attributed to Rev CTD, related to Fig. S7.

Figure 7

(A) Six dimers were extracted from the filament structure to show the adjacent positive difference densities. These cartoons depict the orientation of the Rev subunits in the panels below. The A-A, B-B, and C-C interfaces are shown in blue, orange, and green, respectively, and the ARM is in navy blue. The left and right cartoons are views of the filament interior and exterior, respectively.

(B) Positive difference density is shown (three asymmetric units; contoured at 3σ and with any floating density masked out) in pink, tan and salmon for filaments with acute A-A crossing angles (ӨA-A = ~100°). The density projects out between Rev subunits in a bi-lobed manner. The ordered C-termini from two Rev NTDs (small spheres) co-localize to form an apparent CTD dimerization domain.

(C) Positive difference density is shown, in a similar manner to (B), but for filaments with larger A-A crossing angles (ӨA-A = ~118°). Here, more CTD-attributed density appears to be ordered, forming a U-shaped domain from distinct Rev NTDs.