Table 1.
Primers used in present study
| Primer name | Primer sequence (5′-3′) | Expected size (bp) | GenBank accession number | Purpose |
|---|---|---|---|---|
| pgr-TALEN Forward | AGGAATACGTCGCACACTTT | 965 | Ensembl No. ENSDARG00000035966 | Positive gene Knockout fish screening |
| pgr-TALEN Reverse | CGCCATTTTAATTCGACCTC | |||
| pgr-Forward | GGGTCTCGCTGCGTAATTTT | 841 | Ensembl No. ENSDARG00000035966 | RT-PCR |
| pgr-Reverse | GCCTGGTAGCACTTTCGAAG | |||
| ef1a-Forward | GGCTGACTGTGCTGTGCTGATTG | 409 | BC064291 | Real-time PCR |
| ef1a-Reverse | CTTGTCGGTGGGACGGCTAGG | |||
| fshb-Forward | CAGATGAGGATGCGTGTGC | 281 | AY424303 | Real-time PCR |
| fshb-Reverse | ACCCCTGCAGGACAGCC | |||
| lhb-Forward | GCAGAGACACTTACAACAGCC | 145 | AY424304/AY424305 | Real-time PCR |
| lhb-Reverse | AAAACCAAGCTCTGAGCAGCC |