Figure 5.
Cardiac chymase activity and angiotensin II (Ang II) content in the left ventricles of sham-operated and ovariectomized (OVX) female Fischer 344×Brown Norway (F344BN) rats treated with vehicle (V) or cromolyn for 4 weeks. A) Chymase activity in left ventricles; B) Representative images showing immunohistochemical staining for Ang II in the left ventricles (upper panel), and Ang II staining intensity quantification using ImageJ (lower panel); C) Correlation analysis of cardiac Ang II staining intensity with cardiac chymase activity. ○: sham, ●: OVX-V, Δ: OVX-cromolyn. Values are means ± SEM; *P<0.05 vs. sham. n=5-6/group.