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. Author manuscript; available in PMC: 2017 Jul 17.
Published in final edited form as: J Mol Biol. 2016 May 21;428(14):2814–2831. doi: 10.1016/j.jmb.2016.05.013

Figure 7. Consequence of SSRP1 partial knockdown in LEDGF/p75-deficient cells on HIV-1 infections.

Figure 7

(a and b) LEDGF/p75 wild type or knockout Nalm-6 cells were transduced with a lentiviral vector expressing a Scrambled or SSRP1-specific shRNA and after three days one-third of these cells were analyzed for SSRP1 and LEDGF/p75 expression by immunoblot in (a). The remaining cells were infected with HIV-1-derived viruses expressing luciferase from the HIV-1 LTR (Hluc) or an internal immediate CMV promoter (TRIPluc). Seventy-two hours post-infection, luciferase and viability were measured in (b). (c) LEDGF/p75-deficent (TL3) and control (TC3) cells were transduced with a lentiviral vector encoding a Scrambled or SSRP1-specific shRNA, three days later the cells were infected with Hluc or TRIPluc and seventy-two hours post-infection, luciferase was measured. Luciferase activity measured in the cells transduced with control shRNAs was considered 100% values. Standard deviations represent the variability of six (b) or four (c) independent transduction/infection experiments.