Skip to main content
. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Neuron. 2016 Jun 16;91(1):146–154. doi: 10.1016/j.neuron.2016.05.023

Figure 1. Alkaline sensation does not require G protein signaling.

Figure 1

(A) Alkaline pH evokes an inward current in ASH of wild-type, as well as odr-3(n2150) mutant worms. Bath solution (pH 12) was perfused towards the nose tip where ASH sensory endings are localized. Voltage: −60 mV.

(B) Alkali-activated currents are independent of G protein signaling. Recordings were done as described in (A). Alkaline pH further induced an inward current in the presence of GTPγS (100 μM), while glycerol cannot further evoke a current under the same recording condition (see Figure S1B). GDPβS (500 μM) cannot block alkali-activated current, while it can block glycerol-evoked current under the same recording condition (see Figure S1B). GTPγS and GDPβS were included in the pipette solution and dialyzed into the cell after the formation of whole-cell configuration. Voltage: −60 mV.

(C) Bar graph summarizing the data described in (A–B). n≥8. Error bars: SEM. **p<0.005 (t test).