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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Neuron. 2016 Jun 16;91(1):146–154. doi: 10.1016/j.neuron.2016.05.023

Figure 3. Alkaline pH-induced avoidance behavior requires TMC-1 and OSM-9/TRPV.

Figure 3

(A) Alkaline pH-induced avoidance behavior requires TMC-1 and OSM-9 but not TMC-2. tmc-1(ok1859), osm-9(ky10), and ocr-2(ak47) but not tmc-2(ok1302) mutant worms were severely defective in this behavior. The basal response rate resulted from spontaneous reversals. n=20. Error bars: SEM. **p<0.005, ***p<0.0005 (ANOVA with Dunnett’s test).

(B) TMC-1 and OSM-9 act in ASH neurons to mediate alkaline sensation. Transgenic expression of wild-type tmc-1 and osm-9 cDNA in ASH under the sra-6 promoter rescued the avoidance behavior. n=20. Error bars: SEM. *p<0.05, ***p<0.0005 (ANOVA with Dunnett’s test).

(C) ASH neurons are the primary sensory neurons mediating alkaline pH-induced avoidance behavior. Laser ablation of ASH led to a severe defect in the behavior. n≥20. Error bars: SEM. *p<0.05, **p<0.005 (ANOVA with Dunnett’s test).