Table 2.
Enzyme | Kcat/Km (μM−1 min−1) | Wild-type efficiency (%) |
---|---|---|
WT | 2. 27 ×10−2 | 100 |
P35A | 0.36 ×10−2 | 16.5 |
I36A | 0.36 ×10−2 | 16.6 |
P35A/I36A/I167A | ND | <10 |
Y38A | ND | <10 |
D81A | 3.11 ×10−2 | 136.9 |
I84A | 4.55 ×10−2 | 200.4 |
Y97F | ND | <10 |
L140A | 1.26 ×10−2 | 56.0 |
Y164A | 3.21 ×10−2 | 141.7 |
Y164F | 3.51 ×10−2 | 154.9 |
Y165F | 0.54 ×10−2 | 24.4 |
I167A | 0.74 ×10−2 | 33.3 |