TABLE 3.
Blocking of scFv-induced ADAMTS13 inhibition by rabbit anti-idiotypic IgG
| Rabbit IgG added | % ADAMTS13 activity in presence of scFv inhibitor
|
|||
|---|---|---|---|---|
| scFv 1-416 | scFv 1-420 | scFv 1-428 | scFv 1-431 | |
| none | 20* | 0 | 7* | 19* |
| anti-scFv 1-416 | 93 | 0 | 84 | 13 |
| anti-scFv 1-420 | 0 | 99 | 0 | 0 |
| anti-scFv 1-428 | 75 | 8 | 100 | 72 |
| anti-scFv 1-431 | 70 | 3 | 94 | 92 |
*
Residual ADAMTS13 activities with scFv’s alone are higher than in Fig. 1 because a 2.5-fold lower amount of scFv was used in order to increase sensitivity of blocking scFv by rabbit IgG