Chromatin interactions across the 6q25.1 risk region. (a) Signals
1–5 are numbered and shown as colored stripes. RMND1,
ARMT1, CCDC170 and ESR1
gene structures are depicted with exons (vertical bars) joined by introns
(lines). Gene-enhancer predictions from PreSTIGE12, ChIP-seq binding profiles for
H3K27ac13 and
Encyclopedia of DNA Elements (ENCODE) RNA polymerase II ChIA-PET interactions in
MCF-7 cells are shown. (b–d) 3C anchor points
(3C baits) and interrogated sequences (3C regions) are depicted as black boxes
and gray shading, respectively. 3C interaction profiles in
ER+ MCF-7 and ER− Bre-80 breast cell
lines are shown for signals 1 and 2 (b), signals 3 and 4
(c) and signal 5 (d). 3C libraries were generated
with EcoRI, with the anchor point set at the
ESR1, RMND1-ARMT1 or
CCDC170 promoter region. Graphs present the results from
three biological replicates; error bars, s.d.