Fig. 5.

Alterations of gene expressions in proximal intestine by 8 weeks of Glu supplementation. Relative expression of genes related to nucleic acid synthesis in proximal intestine. A cps2: carbamoyl-phosphate synthetase, A ppat: phosphoribosyl pyrophosphate amidotransferase, C pfas: phosphoribosylformylglycinamidine synthase. The fish were challenged to 8 weeks of experiment. The tissues were collected after the 48 h-fasting. The expression of the genes were estimated by quantitative RT-PCR and normalized by arbp. All values are expressed as the mean ± SEM (n = 8). Different letters indicate significant difference between groups (p < 0.05)