Fig. 3.

Evaluation of the mating abilities of derivative strains. a Schematic outline of the mating assay. Using haploid strains HR42-11T (α-cell) and MC4741 (a-cell) as the mating partners, zygotes were selected on solid medium containing G418, but lacking amino acids (SD/MSG + G418 plates). The Parent strains were Kyokai No. 6, No. 7 and No. 9 (a/α-type cells), and the Derivative strains were K6A, K7A and K9A (isolated as a-type cells) and K6AL, K7AL and K9AL (isolated as α-type cells), respectively. b Images showing colony formation in the mating assay for strains K6A, K7A and K9A. c Images showing colony formation in the mating assay for strains K6AL, K7AL and K9AL. The OD₆₀₀ values of 10-μL cell suspensions spotted on the solid media were set at 1.0, 0.1 and 0.01