Fig. 5.

DNA content histogram of PI-stained yeast cells using FACS. PI-fluorescence data was collected from yeast cells within a gate drawn in the FSC-SSC dot plots (Additional file 1: Fig. S3) according to the data collected from control diploid strains. For relative comparison of DNA content, reference values of 2 N (two sets of all chromosomes) and 4 N (four sets of all chromosomes) were defined as the average of each peak value of Kyokai No.6, No.7 and No.9. The 1 and 8 N values were calculated from the 2 and 4 N values