Figure 8.

KB-R7943 or YM-244769 attenuated the loss of astrocytic mitochondria after OGD. Hippocampal slice cultures with astrocytes expressing mitochondrially targeted EGFP and plasma-membrane-targeted mcherry were fixed in 4% paraformaldehyde 24 h after a 30 min OGD or control treatment. OGD slices were treated 30 min before insult, during the 30 min insult, and during the 24 h recovery period either with no drug, with 15 μm KB-R7943, or with 1 μm YM-244769. A–C, Mean cell density in stratum pyramidale, mitochondrial length in processes (micrometers), and percentage occupancy of processes by mitochondria 24 h after 30 min OGD with and without KB-R7943 or YM-244769. All groups were included in ≥3 experiments, with slice cultures prepared from ≥3 separate animals. n = 8–12 slices/group (2–4 slices/group/experiment, 2 astrocytes/slice, 3 processes/astrocyte). Error bars indicate SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 compared by one-way ANOVA with Bonferroni's correction for multiple comparisons.