Figure 2.

Characterization of the effects of high (23%) versus low (<5%) fat diets on energy consumption, leptin resistance, and adiposity in male and female DBA/2J mice. A, Daily energy consumption (right bars), calculated over a 2 week period, was significantly elevated despite a reduced food intake by mass (left bars). B, In 12 h fasted LCD-fed mice, leptin (1 mg/kg i.p.) significantly reduced 3 h food intake; whereas in HCD-fed mice, there was no response to leptin, indicating that they had become resistant to this hormone. Serum leptin concentration (C) and regional (gonadal, inguinal, and retroperitoneal) fat pad masses (D) were markedly increased in male, and to a lesser extent in female, mice in response to the HCD. Mice (n = 6 per group) were 5–6 months old and were fed the treatment diets for 12 weeks. *p < 0.05 versus LCD controls. **p < 0.01 versus LCD controls.