Table 1.

Primer sequence, annealing temperature (T°), and annealing time for each measured gene using quantitative real time PCR (qPCR)

Parameters	Primer sequence	Annealing T° (°C)	Annealing time (s)
IL-6 (358 bp)	Forward primer: GCC TTC GGT CCA GTT GCC TT	60	24
	Reverse primer: GCA GAA TGA GAT GAG TTG TC	60	24
ICAM-1 (406 bp)	Forward Primer: AGAGGTCTCAGAAGFGGACCG	58	45
	Reverse Primer: GGGCCATACAGGACACGAAG	58	45
VCAM-1 (460 bp)	Forward primer: GGTGGGACACAAATAAGGGTTTTGG	60	45
	Reverse primer: CTTGCAATTCTTTTACAGCCTGCC	60	45
E-selectin (244 bp)	Forward primer: TGAAGCTCCCACTGAGTCCAA	60	45
	Reverse primer: GGTGCTAATGTCAGGAGGGAGA	60	45
TNF-α (84 bp)	Forward primer: CCGGGCGTGGTGGTGAG	60	45
	Reverse primer: TCTGCCTTTTGGGTCTTGTGAATA	60	45
NFκB (p50) (376 bp)	Forward primer: AAC CTG CAG ACT CCA CT	65	45
	Reverse primer: ACA CCA GGT CAG GAT TTT GC	65	45
eNOS (418 bp)	Forward primer: ATGGGCAACTTGAAGAGCGTGG	55.8	45
	Reverse primer: TAGTACTGGTTGATGAAGTCCC	55.8	45
HPRT-1 (523 bp)	Forward primer: GGCAAAACAATGCAAACCTT	60	45
	Reverse primer: CAAGGGCATATCCTACGACAA	60	45
GAPDH (472 bp)	Forward primer: CCACCCATGGCAAATTCCATGGCA	60	45
	Reverse primer: TCTAGACGGCAGGTCAGGTCCACC	60	45