Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 8;16:431. doi: 10.1186/s12885-016-2468-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — Involvement of DNA methylation in E-cadherin 5′ region. a Demethylation agent 5′AZA could restored E-cadherin expression on A253-5 cells. b Illustration of CpG islands distribution on 5′ region of E-cadherin. c Methylation specific PCR of E-cadherin in A253 cells. M indicated methylated amplicon and U indicated unmethylated amplicon. MSS1, positive control; WBC: normal human white blood cell for negitive control. d Bissulfite sequence of E-cadherin in A253 cells reveal that knockdown of DNMT 3B could result in demethylation of 5′ region of E-cadherin. *** indicated p < 0.001 as compared with A253-0 and ### indicated p < 0.001 as compared with A253-5