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Kinetics of hydrolysis of aryl-monosaccharides by THSAbf
| Substrate | K M (mM) | k cat (s−1) | k cat/K M (s−1 mM−1) | SA (U mg−1) |
|---|---|---|---|---|
| pNP-Araf | 0.31 ± 0.1 | 328 ± 23 | 1050 | 426 ± 36 |
| pNP-Xylp | 23.8 ± 4.1a | 10.6 ± 0.9 | 0.44 | 1.70 ± 5 |
| pNP-Galf | 8.6 ± 0.9a | 327 ± 20 | 38 | 103 ± 2 |
aThese values are subject to caution since the maximum substrate concentration used in each case was less than 2 × K M. n = 3 and SD values are shown
