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. Author manuscript; available in PMC: 2016 Dec 13.
Published in final edited form as: Nat Struct Mol Biol. 2016 Jun 13;23(7):682–690. doi: 10.1038/nsmb.3248

Figure 6. Overexpression of NPC-enriched PRC1 subunits can affect genomic localization of Ring1b.

Figure 6

(a) Stoichiometry values for Cbx proteins bound to Ring1b in mock or Cbx4-transfected Ring1b-GFP BAC ESCs. Data are shown as mean ± s.d. (n = 3 pulldowns) (b) Western blots of Ring1b co-immunoprecipitations in mock or Pcgf4-transfected ESCs. Uncropped blots appear in Supplementary Data Set 1. (c-d) ChIP-qPCR of Ring1b, IgG, and histone H3 at 2 NPC-enriched loci (c) and at 2 ESC-enriched loci (d) in mock, Cbx4 or Pcgf4-transfected ESCs. Data shown are from one representative ChIP (n = 3 ChIPs) and graphed as mean ± s.d. (n = 3 technical replicates). (e) qRT-PCR analysis of gene expression levels in mock, Cbx4 or Pcgf4-transfected ESCs. Data shown are from one representative transfection (n = 3 transfections) and graphed as mean ± s.d. (n = 3 technical replicates).