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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Neurosci Lett. 2016 May 26;627:77–83. doi: 10.1016/j.neulet.2016.05.055

Figure 2. Effect of Gγ7 silencing 120 hr post siRNA transfection on the Noc- and NE-mediated Ca2+ current inhibition in SG neurons.

Figure 2

A and B) Time courses of peak Ca2+ current amplitude inhibition for pre- (●) and postpulse (○) acquired from the sequential application of Noc (1 μM) and NE (10 μM) in neurons transfected with control scrambled (A) and Gγ7 (B) siRNA, respectively. Superimposed Ca2+ current traces (shown to the right) evoked with the `triple-pulse' voltage protocol (shown on top of 1A) in the absence (1 and 2; 5 and 6, black) or presence (3 and 4; 7 and 8, grey) of either NE or Noc. Currents were evoked every 10 sec. The filled bars indicate the application of agonists. C) Summary graph of mean (± SE) Ca2+ current inhibition produced by application of Noc or NE in neurons transfected with scrambled or Gγ7 siRNA. Inhibition was determined from the Ca2+ current amplitude measured isochronally at 10 msec into the prepulse (+10 mV) in the absence or presence of Noc or NE. Numbers in parenthesis indicate the number of neurons tested. * P < 0.05 compared to neurons transfected with scrambled siRNA, Student's t test.