Sf-rhabdovirus-like sequences are present in and transcribed from Sf cell genomes. A) The relative sizes and positions of the newly found N-, P-, G-, and L-like ORFs as compared to their homologs in the Sf-rhabdovirus genome, and the position of a 320 nt deletion found in some Sf-rhabdovirus sequences. PCR demonstrating presence and transcription of Sf-rhabdovirus N- (B), P- (C), G- (D) and L-like (E) sequences in Sf9 and Sf-RVN cells. Expected PCR amplimer sizes are indicated. Templates were genomic DNA (gDNA), reverse-transcribed total cellular RNA (cDNA), mock reverse-transcribed total cellular RNA (−RT) or no template (H2O).