Table 1.
The effect of EMD 386088 on the concentration of DA and its metabolites in rat brain structures
| Treatment | (mg/kg) | DA (ng/g t) | DOPAC (ng/g t) | 3-MT (ng/g t) | HVA (ng/g t) |
|---|---|---|---|---|---|
| Hippocampus | |||||
| Vehicle | 0 | 42 ± 10 | 9 ± 1.9 | 1.0 ± 0.5 | 14 ± 1.7 |
| EMD 386088 | 2.5 | 58 ± 4 | 5 ± 0.6 | 1.5 ± 0.4 | 12 ± 0.9 |
| 5 | 51 ± 4 | 5 ± 0.5 | 1.3 ± 0.6 | 13 ± 0.7 | |
| F(2, 20) = 1.25 NS |
F(2, 20) = 2.9 NS |
F(2, 21) = 0.21 NS |
F(2, 21) = 0.47 NS |
||
| Nucleus accumbens | |||||
| Vehicle | 0 | 9756 ± 777 | 2066 ± 181 | 139 ± 18 | 1195 ± 102 |
| EMD386088 | 2.5 | 9586 ± 389 | 1722 ± 73 | 169 ± 20 | 849 ± 41** |
| 5 | 10,017 ± 505 | 1614 ± 79* | 143 ± 6 | 843 ± 39** | |
| F(2, 21) = 0.14 NS |
F(2, 21) = 3.76 p < 0.05 |
F(2, 21) = 1.02 NS |
F(2, 21) = 8.82 p < 0.01 |
||
| Striatum | |||||
| Vehicle | 0 | 11,386 ± 325 | 1773 ± 61 | 336 ± 7 | 1487 ± 111 |
| EMD386088 | 2.5 | 11,305 ± 298 | 1589 ± 52* | 329 ± 11 | 1228 ± 7 9* |
| 5 | 11,253 ± 327 | 1430 ± 47** | 332 ± 12 | 1180 ± 66* | |
| F(2, 21) = 0.33 NS |
F(2, 21) = 8.76 p < 0.01 |
F(2, 21) = 1.53 NS |
F(2, 21) = 5.06 p < 0.05 |
||
EMD386088 was injected i.p. in a dose of 2.5 and 5 mg/kg; control group was treated i.p. with saline. The rats were decapitated about 30 min after drug injection. The results are expressed as the mean ± SEM (N = 8). The indices were calculated using concentrations from individual tissue samples. The concentration of dopamine and its metabolites were measured in ng/g tissue. The data were analyzed by means of one-way ANOVA, followed by Duncan’s post hoc test
NS not significant
Statistical significance: *p < 0.05, **p < 0.01 vs. respective control group