Table 2.

The effect of EMD 386088 administration on rate of DA metabolism in rat brain structures

Treatment	(mg/kg)	[DOPAC]/[DA]	[3-MT]/[DA]	[3-MT]/[DOPAC]	[HVA]/[DA]
Hippocampus
Vehicle	0	28 ± 5.1	1.7 ± 0.6	8 ± 2.8	56 ± 14.2
EMD386088	2.5	9 ± 1.2**	2.7 ± 0.9	27 ± 7.4*	22 ± 1.6*
	5	10 ± 1.0**	2.0 ± 1.3	23 ± 16.3	27 ± 2.1*
		F(2, 20) = 11.03 p < 0.001	F(2, 20) = 0.28 NS	F(2, 20) = 4.11 p < 0.05	F(2, 20) = 4.53 p < 0.01
Nucleus accumbens
Vehicle	0	21 ± 0.7	1.4 ± 0.1	7 ± 0.3	12 ± 0.8
EMD386088	2.5	18 ± 0.4**	1.7 ± 0.2	10 ± 0.9.**	9 ± 0.4**
	5	16 ± 0.7**	1.4 ± 0.1	9 ± 0.1*	8 ± 0.5**
		F(2,21) = 18.3 p < 0.0001	F(2, 21) = 2.52 NS	F(2, 21) = 6.81 p < 0.01	F(2, 21) = 14.54 p < 0.0001
Striatum
Vehicle	0	15 ± 0.3	2.9 ± 0.1	19 ± 0.6	13 ± 0.7
EMD386088	2.5	14 ± 0.4*	3.3 ± 0.1	23 ± 0.5**	10 ± 0.7*
	5	13 ± 0.4**	2.9 ± 0.1	23 ± 0.7**	10 ± 0.6*
		F(2, 21) = 13.99 p < 0.0001	F(2, 21) = 3.88 p < 0.05	F(2, 21) = 13.68 p < 0.0001	F(2, 21) = 4.35 p < 0.05

EMD386088 was injected i.p. in a dose of 2.5 and 5 mg/kg; control group was treated i.p. with saline. The rats were decapitated about 30 min after drug injection. The results are expressed as the mean ± SEM (N = 8). The rate of DA total metabolism was expressed as the ratio [HVA] / [DA] × 100; the rate of DA MAO-dependent oxidation as the ratio: [DOPAC] / [DA] × 100; the rate of the inhibition of dopamine reuptake as the ratio: [3-MT] / [DOPAC] × 100; and the rate of COMT-dependent O-methylation as the ratio: [3-MT] / [DA] × 100. The indices were calculated using concentrations from individual tissue samples. The data were analyzed by means of one-way ANOVA, followed by Duncan’s post hoc test

NS not significant

Statistical significance: *p < 0.05, **p < 0.01 vs. respective control group