Table 3.
The effect of EMD 386088 administration on 5-HT and its metabolite concentration and 5-HT metabolism in rat brain structures
| Treatment | (mg/kg) | 5-HT (ng/g t) | 5-HIAA (ng/g t) | [5-HIAA]/[5-HT] |
|---|---|---|---|---|
| Hippocampus | ||||
| Vehicle | 0 | 275 ± 11 | 168 ± 15 | 61 ± 5 |
| EMD386088 | 2.5 | 294 ± 11 | 185 ± 9 | 63 ± 2 |
| 5 | 288 ± 11 | 173 ± 15 | 59 ± 4 | |
| F(2, 21) = 0.79 NS |
F(2, 21) = 0.44 NS |
F(2, 21) = 0.22 NS |
||
| Nucleus accumbens | ||||
| Vehicle | 0 | 481 ± 42 | 324 ± 21 | 69 ± 5 |
| EMD386088 | 2.5 | 551 ± 16 | 354 ± 14 | 64 ± 3 |
| 5 | 435 ± 31 | 298 ± 20 | 69 ± 3 | |
| F(2, 21) = 3.44 NS |
F(2, 21) = 2.17 NS |
F(2, 21) = 0.41 NS |
||
| Striatum | ||||
| Vehicle | 0 | 369 ± 19 | 358 ± 22 | 97 ± 4 |
| EMD386088 | 2.5 | 381 ± 13 | 392 ± 16 | 103 ± 3 |
| 5 | 346 ± 18 | 352 ± 18 | 103 ± 6 | |
| F(2, 21) = 1.14 NS |
F(2, 21) = 1.26 NS |
F(2, 21) = 0.54 NS |
||
EMD386088 was injected i.p. in a dose of 2.5 and 5 mg/kg; control group was treated i.p. with saline. The rats were decapitated about 30 min after drug injection. The results are expressed as the mean ± SEM (N = 8). The concentration of serotonin (5-HT) and its metabolite, 5-hydroxyindolacetic acid (5-HIAA), was expressed as ng/g tissue. The rate of 5-HT metabolism was expressed as the ratio [5-HIAA] / [5-HT] × 100. The indices were calculated using concentrations from individual tissue samples. The data were analyzed by means of one-way ANOVA, followed by Duncan’s post hoc test
NS not significant