Table 4.

The effect of EMD 386088 administration on NA and its metabolite concentration and NA metabolism in rat brain structures

Treatment	(mg/kg)	NA (ng/g t)	NM (ng/g t)	[NM]/[NA]
Hippocampus
Vehicle	0	312 ± 15	14 ± 1.3	5 ± 0.4
EMD386088	2.5	324 ± 16	16 ± 0.9	5 ± 0.3
	5	300 ± 22	15 ± 1.6	5 ± 0.4
		F(2, 21) = 0.46 NS	F(2, 21) = 0.45 NS	F(2, 21) = 0.31 NS
Nucleus accumbens
Vehicle	0	443 ± 48	15 ± 1.0.8	4 ± 0.1
EMD386088	2.5	443 ± 40	14 ± 0.8	4 ± 0.2
	5	445 ± 47	13 ± 0.8	5 ± 0.1
		F(2, 21) = 0.01 NS	F(2, 21) = 0.0 NS	F(2, 21) = 0.99 NS

EMD386088 was injected i.p. in a dose of 2.5 and 5 mg/kg; control group was treated i.p. with saline. The rats were decapitated about 30 min after drug injection. The results are expressed as the mean ± SEM (N = 8). The concentration of NA and its metabolite, normetanephrine (NM), was expressed as ng/g tissue. The rate of NA metabolism was expressed as the ratio of the extraneuronal metabolite NM to NA: [NM] / [NA] × 100. The indices were calculated using concentrations from individual tissue samples. The data were analyzed by means of one-way ANOVA, followed by Duncan’s post hoc test

NS not significant