Fig. 1.

Effect of increasing inoculum dose of live K. pneumoniae NCTC9633 (a) or NCTC13438 (c) and E. coli NCTC12241 (b) or NCTC13353 (d) on the survival of G. mellonella larvae during incubation at 37 °C for 96 h. Numbers in the legend indicate the inoculum size in cells per larva. For all strains, the effect of heat-killed (h.k.) bacterial inocula is also shown. No significant mortality was observed in an unmanipulated group (data not shown) or in the uninfected group sham-infected with sterile PBS. With all treatment groups not labelled with tilde, survival was significantly reduced compared to the group sham-infected with PBS (p < 0.05, log-rank test with Holm correction for multiple comparisons); n = 45 (pooled from replicate experiments)