Figure 4.

Cell viability of primary Müller cells in response to starvation and treatment with glutamate. By means of MTT assays survival of starved Müller cells significantly decreased to 22% (^∗∗∗∗ P < 0.0001) compared with control. Additional treatment with 1 mM glutamate in combination with starvation also reduced the Müller cell viability to 39% (^∗∗∗∗ P < 0.0001). Nevertheless, treatment with glutamate enhanced Müller survival significantly compared to solely starved Müller cells (^∗ P < 0.05). The bars represent mean values ± SEM of 3 experiments. Differences in survival were tested using one-way ANOVA followed by Tukey's multiple comparisons test.