Figure 5.

Glutamate uptake in starved Müller cells. Müller cells were cultured 24 hours in energy sufficient conditions or starved within the same time frame. Kinetic L-glutamate uptake at extracellular glutamate concentrations from 1 to 500 μM was measured during the described conditions. Starvation significantly increased glutamate uptake ((a); ^∗ P < 0.05 and ^∗∗ P < 0.01. P = 0.067 at 500 μM glutamate). The relative maximal uptake rate, V _max, was also significantly increased ((b); P < 0.05). Values are represented as mean values ± SEM of 5 experiments. Differences were tested using unpaired two-tailed t-test at the different glutamate concentrations.