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Validation of the optimized HPLC-UV conditions for three analytes.
| Iridoid | Calibration curve | Correlation (R 2) | Precision (RSD%) |
Reproducibility (RSD%) |
Stability (RSD%) |
Recovery (%) |
LLOD (mg/mL) |
|---|---|---|---|---|---|---|---|
| Catalpol | y = 0.9707x + 6.7723 | 0.9997 | 0.14 | 0.25 | 1.46 | 102.59 | 1.3333E − 04 |
| Aucubin | y = 0.9952x + 6.8458 | 0.9999 | 2.65 | 3.08 | 2.38 | 98.29 | 1.8170E − 04 |
| GPA | y = 1.0136x + 7.5797 | 0.9996 | 2.05 | 2.41 | 0.43 | 101.96 | 4.9926E − 05 |
