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. Author manuscript; available in PMC: 2016 Jul 10.
Published in final edited form as: J Biol Rhythms. 2015 Jul 6;30(5):396–407. doi: 10.1177/0748730415593377

Figure 1.

Figure 1

L oss of Kv1.4 or Kv4.2 shortens the circadian period of clock gene expression in isolated suprachiasmatic nucleus (SCN). PERIOD2::LUCIFERASE (PER2::LUC) bioluminescence was measured in vitro in SCN slices prepared from mice expressing the PER2::LUC reporter construct in place of the endogenous mouse Per2 (Yoo et al., 2004) and lacking Kv1.4, Kv4.2, Kv4.3, or both the Kv1.4 and Kv4.2 α subunits (see the Materials and Methods section). (A–E) Representative examples of PER2::LUC bioluminescence are shown as double-plotted actograms and normalized to their daily peak in Per2Luc (A), Kv1.4−/−Per2Luc (B), Kv4.3−/−Per2Luc (C), Kv4.2−/−Per2Luc (D), and Kv1.4−/−/Kv4.2−/−Per2Luc (E) slices. In each plot, the linear regression through the daily peaks illustrates the intrinsic period of the slice. In (F), the average periods (gray lines) of PER2::LUC expression in SCN slices from Kv1.4−/−Per2Luc (n = 18), Kv4.2−/−Per2Luc (n = 11), and Kv1.4−/−/Kv4.2−/−Per2Luc (n = 12) were significantly (*p < 0.02 1-way analysis of variance) shorter than in SCN slices from Per2Luc (n = 15) or Kv4.3−/−Per2Luc (n = 14) animals. Open circles represent individual SCN slice values; error bars show SEM.