Additive effect of curcumin and genistein on G551D-CFTR in cell-attached mode. (A) Representative traces for the additive effect of curcumin and genistein on G551D-CFTR single channel currents in cell-attached mode. The G551D-CFTR channels, expressed in CHO cells, are continuously stimulated by 10 μM FSK and 100 μM CPT-cAMP. Vp=+50 mV. Single channel amplitude: control: 0.27 pA, genistein: 0.35 pA, genistein+curcumin: 0.30 pA, wash out: 0.31 pA. (B) Comparisons of relative fold-increase in NPo of G551D-CFTR single channel induced by 80 μM genistein alone and a combined application of 80 μM genistein and 30 μM curcumin (n=10). Error bars represent SEM. Paired t-test, **: P<0.01.