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. Author manuscript; available in PMC: 2016 Jul 10.
Published in final edited form as: Methods Mol Biol. 2011;741:419–441. doi: 10.1007/978-1-61779-117-8_27

Fig. 27.3. Monte Carlo simulation of the effects of pyrophosphate on macroscopic CFTR Cl− currents.

Fig. 27.3

(a) A kinetic model to simulate the reopening of macroscopic CFTR Cl− currents induced by pyrophosphate (PPi). In this model, C0, C1 and C2 are closed states with zero, one and two ATP molecule bound, respectively, while Ci is an intermediate closed state sensitive to PPi. O, OiPPi and O0PPi are open states, with the latter two open states induced by PPi binding from the Ci and C0 states, respectively. The rate constants describing transitions between the different states are shown. (b) Comparison of (a) simulated and (b) experimental macroscopic current data for the PPi-induced reopening of CFTR channels after removal of ATP. Double exponential fits to current relaxations after removal of PPi are denoted by continuous and dashed lines in the simulated (a) and experimental (b) data, respectively. Time constants: (a) τ fast: 1.7 s, τ slow: 32.1 s; (b) τ fast: 1.9 s, τ slow: 32.4 s. For further information, see (36). Modified from The Journal of General Physiology 2009, 133:405–419. Copyright 2009 The Rockefeller University Press.