| pCR2.1 |
Cloning vector, CarbR, KmR
|
Invitrogen |
| pRDH10 |
ori(R6K) mobRP4 sacRB TetR CmR
|
Lab stock |
| pBS34 |
pBluescript II KS+, KSAC cassette, CarbR, KmR
|
29 |
| pWSK29 |
Cloning vector, ori(pSC101) CarbR
|
Lab stock |
| pFF35 |
5′ and 3′ flanking regions of gudTygcYgudDSTM2959 operon in pCR2.1, CarbR, KmR
|
This Study |
| pFF57 |
KSAC cassette flanked by up-/downstream regions of the gudTygcYgudDSTM2959operon in pRDH10; CmR, KmR
|
This Study |
| pSW172 |
ori(R101) repA101ts CarbR
|
30 |
| pCAL61 |
ori(R101) KanR StrepR
|
12 |
| pCAL62 |
ori(R101) CarbR StrepR
|
12 |
| pFF62 |
Up-/down stream regions of gudD from IR715 in pRDH10 |
This Study |
| pFF63 |
Up-/downstream regions of garD from IR715 in pRDH10 |
This Study |
| pFF64 |
Up-/downstream regions of the gudDXP operon from EcN in pRDH10 |
This Study |
| pFF65 |
Up-/downstream regions of garD from EcN in pRDH10 |
This Study |
| pGUDT |
gudTygcYgudDSTM2959 operon under the control of its native promoter in pWSK29; CarbR
|
This Study |
