Extended Data Table 5. Primers used in this study.

Deletion of gudTygcYgudDSTM2959
Primer	Sequence*
71	5′-GGATCCTCTGAACCGCTGCTAATGG-3′
72	5′-TCTAGAGTTACGCTGAGTTGTAGG-3′
73	5′-TCTAGAGTAGGGAATCAGAGATAACG-3′
74	5′-GGATCCAGGGAGATACGCATAATGG-3′
Deletion of gudD in IR715
116	CACACCCGTCCTGTGCTCAACATGCACGATTCG
117	CGATCTCCCGATGTTTACCCATGTTG
118	TAAACATCGGGAGATCGAACGTTTGTAAG
119	GCGTCCGGCGTAGAGGAGCTTGACTGGGAACAG
Deletion ofgarD in IR715
112	CACACCCGTCCTGTGCTGAAATCAGAATGGGTC
113	TCACAGGTCGGAATATGTTCAGTCAGTTC
114	CATATTCCGACCTGTGACCTGATCTATTCTG
115	GCGTCCGGCGTAGAGATTGTCGCAAGGCTTCAC
Complementation of gudTygcYgudDSTM2959
92	5′-TCCTGCAGCCCGGGGCTTGCGTTGCCAGTAAGC-3′
93	5-TTAACGCACCATGCAAGGAC-3′
94	5′-CTTGCATGGTGCGTTAAGTC-3′
95	5′-CGCTCTAGAACTAGTGATCCGGCCTACAACTCAGC-3′
Deletion of gudDXP operon in E. coli Nissle 1917
136	CACACCCGTCCTGTGCTGTGTTTATGCCGGATG
137	CCGGTTCGTTCCCTGGCGATGTTTAC
138	CCAGGGAACGAACCGGCAATAGAAAGC
139	GCGTCCGGCGTAGAGTTTGCCTGGAGTCAAGCG
Deletion ofgarD in E. coli Nissle 1917
235	CACACCCGTCCTGTGTGGCCAACATCAAAATCAG
236	CACCGGTGGTTCGGGTATTTCGGTAG
237	ACCCGAACCACCGGTGACCTGATTTC
238	GCGTCCGGCGTAGAGGCCAGCGACAAGTTTCTTTC
Quantitative real-time RT-PCR
Organism	Target	Sequence
Mus musculus	B2M	5′-GGTCTTTCTGGTGCTTGTCTCA-3′
		5′-GTTCGGCTTCCCATTCTCC-3′
Mus musculus	Nos2	5′-TTGGGTCTTGTTCACTCCACGG-3′
		5′-CCTCTTTCAGGTCACTTTGGTAGG-3′

^*restriction enzyme sites are underlined, overlapping sequences for Gibson Assembly are in bold