Extended Data Figure 10. Reversibility of UPR^mt-induced mitochondrial pre-RNA processing and translation defects.

a, Coomassie gel staining as a loading control of the same experiment as in Fig. 4e. b, Mitochondrial pre-RNA processing was measured by qPCR in cells subjected to GTTP pulse-chase wash-out for 1-4h. Data are averages of fold changes versus untreated ±s.d., two-tailed p-values *p≤0.05, **p≤0.01, ***p≤0.001, n=3 biological replicates. c, Analysis of mitochondrial translation in wild-type or MRPP3 overexpressing cells with or without GTPP treatment. Newly synthesized proteins were labeled with ³⁵S and analyzed by phospho-imager. d, Immunoblot of TFB1M expression with or without 6h GTPP treatment (left). Quantification of control normalized TFB1M levels from immunoblots of two independent experiments.