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ECM protein conjugation on polyacrylamide (PAAm) substrates. (a–c) A scheme is shown for ECM protein conjugation using sulfo-SANPAH (1). This reaction targets all available amines, resulting in heterogeneous protein attachment. (d–f) In this work, protein N-termini are coupled to 2-pyridinecarboxaldehyde (2PCA)-modified PAAm gels, resulting in a more uniform presentation. (g) A 2PCA derivative for acrylamide copolymerization is shown. Protein N-termini are shown in blue in (b) and (f).
