Fig. 2.
2PCA conjugation of ECM proteins in solution. The N-terminal modification of type I collagen (a, b), fibronectin (c, d) and laminin (e, f) with 2PCA-fluorescein was monitored by SDS-PAGE. The SDS-PAGE gels were fluorescently imaged (b, d, f) to visualize conjugated products and Coomassie stained (a, c, e) to show total protein content. Benzylalkoxyamine (benzyl-ONH2) and fluorescein were used as controls, which show minimal fluorescence. Arrows indicate corresponding ECM protein subunits.