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. 2016 Jun 23;9:57–66. doi: 10.1016/j.redox.2016.06.003

Fig. 2.

Fig. 2.

4-HNE inhibits oxidative burst and glycolysis in human neutrophils. Representative profiles of PMA-stimulated (A) oxygen consumption rate and (C) extracellular acidification rate of neutrophils pre-treated with 4-HNE at the indicated concentration for 2 h using the extracellular flux analyzer are shown. The area under the curve of OCR (B) and ECAR (D) following PMA stimulation in 4-HNE or alkyne HNE (aHNE) treated neutrophils. The IC50 of 4-HNE and aHNE for OCR and ECAR was calculated from 3 independent samples (shown in Figs. 2B & 2D). Mean±sem from n=5–6 replicates with an individual representative donor. #, p≤0.05 compared to the respective untreated control.