Table 2.
Cellular metabolism-related proteins are targets of aHNE in human neutrophils. Metabolic proteins that are modified by aHNE were identified by mass spectrometry. Table demonstrates the list of proteins with protein accession ID, number of peptides identified with 95% confidence, and the percent coverage of the peptides identified to the sequence of the protein with 95% confidence are shown.
| No | Protein name | Accession | Peptides | % Coverage |
|---|---|---|---|---|
| 1 | Alpha-enolase | P52209 | 18 | 48.24 |
| 2 | Pyruvate kinase isozymes M1/M2 | P06733 | 33 | 72.12 |
| 3 | 6-phosphogluconate dehydrogenase, decarboxylating | P04075 | 7 | 42.58 |
| 4 | L-lactate dehydrogenase A chain | P11413 | 11 | 54.17 |
| 5 | Transketolase | P06744 | 12 | 39.07 |
| 6 | Phosphoglycerate kinase 1 | P06737 | 11 | 26.09 |
| 7 | Glucose-6-phosphate isomerase | P52790 | 9 | 25.35 |
| 8 | Glucose-6-phosphate 1-dehydrogenase | P00338 | 16 | 44.58 |
| 9 | Glycogen phosphorylase, liver form | P07195 | 6 | 23.05 |
| 10 | Transaldolase | P40925 | 2 | 12.87 |
| 11 | Hexokinase-3 | Q96G03 | 2 | 18.95 |
| 12 | Triosephosphate isomerase | P00558 | 14 | 36.21 |
| 13 | Fructose-bisphosphate aldolase A | P00491 | 4 | 28.72 |
| 14 | L-lactate dehydrogenase B chain | P14618 | 30 | 68.36 |
| 15 | UTP--glucose-1-phosphate uridylyltransferase | P37837 | 10 | 43.62 |
| 16 | Purine nucleoside phosphorylase | P29401 | 15 | 45.75 |
| 17 | Malate dehydrogenase, cytoplasmic | P60174 | 8 | 41.37 |
| 18 | Phosphoglucomutase-2 | Q16851 | 5 | 29.72 |