Figure 3.

AFIF's action on PCSMS contraction amplitude is negatively correlated with Ca²⁺ concentration. n = 10, ^b P < 0.01 versus Ca²⁺-normal environment. AFIF's action on PCSMS was most obvious in low Ca²⁺ environment, and the high concentration of Ca²⁺ weakened the diastolic effect of AFIF on PCSMS. When AFIF concentration reached 10000 μg/mL, the PCSMS contraction amplitude in low Ca²⁺ environment was significantly lower than Ca²⁺-normal environment (P < 0.01), while the PCSMS contraction amplitude in high Ca²⁺ environment was significantly higher compared with the Ca²⁺-normal environment (P < 0.01).