Fig. 2.

Artemisinin attenuated H₂O₂-induced mitochondrial membrane potential (△ψm) loss and caspase 3/7 activity increase in D407 cells. After pre-treatment with 30 μM Artemisinin or 0.1% DMSO (vehicle control) for 2 h, D407 cells were incubated with or without 100 μM H₂O₂ for another 24 h. (A) △ψm was determined by the JC-1 assay. (B) Quantification of caspase 3/7 activity was determined by caspase 3/7 activity assay. ^###P<0.005 versus control group;***P<0.005 versus H₂O₂-treated group was considered significantly different.